Publications by Dr. Pierre Vanderzwalmen

1. Scientific publications

MASSIP A, VANDERZWALMEN P, ECTORS F, DE COSTER R, D’IETEREN G and HANZEN C Deep freezing of cattle embryos in glass ampules or french straws. Theriogenology, 1979, 12, 79-84.

MASSIP A and VANDERZWALMEN P In vtro survival of mouse embryos frozen in glycerol or glycerol-sucrose. Cryo-letters, 1982, 3, 326.

BECKERS J.F., FROMONT-LIENARD Ch., VANDERZWALMEN P., WOUTERS- BALLMAN P.and ECTORS F. Isolement d’une hormone placentaire bovine présentant une activité analogue à la prolactine et à l’hormone de croissance. Ann. Méd.Vét. 1980, 124, 585-601.

CONSTANTINESCU M.D., MASSIP A. and VANDERZWALMEN P. Research with a view of improvement of non surgical embryo sampling technique in cattle. Simpzionul ameliorarea, technologia si patologia rumegatoarelor Cluj-Napoca, 1981 pp 83-90.

MASSIP A., MULNARD J., HUYGEN R., HANZEN C., VANDERZWALMEN P. and ECTORS F. Ultrastructure of the cow blastocyst. J. Submicrosc. Cytol., 1981, 13, 31-40.

BECKERS J.F., DE COSTER R., WOUTERS-BALLMAN P., FROMONT-LIENARD Ch, VANDERZWALMEN P.and ECTORS F. Dosage radioimmunologique de l’hormone placentaire somatotrope et mammotrope bovine. Ann. Méd. Vét., 1982, 126, 9-21.

MASSIP A., MULNARD J., VANDERZWALMEN P., HANZEN C. and ECTORS F. The behaviour of cow blastocyst in vitro : cinematographic and morphometric analysis. J. Anat., 1982, 134, 399-405.

MASSIP A., VANDERZWALMEN P., HANZEN C. and ECTORS F. Fast freezing of cow embryos in french straws with an automatic program. Theriogenology, 1982, 18, 325-332.

MASSIP A., VANDERZWALMEN P., MULNARD. and ZWIJSEN W. Atypical hatching of a cow blastocyst leading to separation of complete twin half blastocysts. Vet. Rec., 1983, 112, 301.

LEROY F., MASSIP A., PUISSANT., CAMUS M. and VANDERZWALMEN P. Preliminary data on an animal model for the study of combined in vitro fertilization and embryo storage. In Recent progress in human in vitro fertilization. 1983, 303-309. Eds W Feichtinger and P Kemeter

MASSIP A., VANDERZWALMEN P., PUISSANT F., CAMUS M. and LEROY F. Effect of in vitro fertilization, culture, freezing and transfer on the ability of mouse embryos to implant and survive. J. Reprod. Fert.,1984, 71, 199-204.

MASSIP A., VANDERZWALMEN P. and LEROY F. Effect of stage of development on survival of mouse embryos frozen-thawed rapidly. Cryobiology, 1984, 21, 574-577.

MASSIP A. and VANDERZWALMEN P. Direct transfer of frozen cow embryos in glycerol-sucrose. Theriogenology, 1982, 18, 325-332.

MASSIP A., VANDERZWALMEN P. and ECTORS F. Production de jumeaux monozygotes par duplication d’embryons chez les bovins. Ann. Méd.Vét.,1985, 129, 53-57.

MASSIP A. and VANDERZWALMEN P. La duplication des embryons chez la vache. Vlaams Diergeneeskundig Tijdschrift, 1986, 55, 63-74.

SCHEFFEN B., VANDERZWALMEN P and MASSIP A A simple and efficient procedure for preservation of mouse embryos by vitrification. Cryo-letters, 1986, 7, 260-269.

MASSIP A., VANDERZWALMEN P, SCHEFFEN B. and ECTORS F. Pregnancies following transfer of cattle embryos preserved by vitrification. Cryo-letters, 1986, 7, 270-273.

MASSIP A., VANDERZWALMEN P. and ECTORS F.

Recent progress in cryopreservation of cattle embryos. Theriogenology, 1987, 1, 69-79.

BECKERS J.F., DEMOUTIER M., VANDERZWALMEN P., BALLMAN P., MASSIP. A., and ECTORS F. Isolation and use of FSH to increase the quality of superovulation in cattle. Theriogenology, 1987, 1, 213.

MASSIP A., VANDERZWALMEN P. and ECTORS F. Cryopréservation de l’embryon de bovin: techniques et résultats. Ann. Méd. Vét., 1987, 131, 515-528.

MASSIP A. and VANDERZWALMEN P Développement de quarts d’embryons bovins frais ou après vitrification. Ann. Méd. Vét. 1988, 132, 483-487.

DEMOUSTIER M., BECKERS J.F., VANDERZWALMEN P., CLOSSET J. and ECTORS F. Determination of Porcine plasma follitropin levels during superovulation treatment in cours. Theriogenology 1988, 6, 674.

ECTORS F.J., VANDERZWALMEN P., TOUATI.K., BECKERS J.F. and ECTORS F. Obtention de blastocystes après maturation et fécondation in vitro d’ovocytes de bovin: premiers résultats. Ann. Méd.Vét, 1988, 132, 517-519.

ECTORS F.J., VANDERZWALMEN P., TOUATI K., BECKERS J.F. and ECTORS F. In vitro maturation and fertilization of bovine oocytes. Theriogenology 1989, 31, 188.

VANDERZWALMEN P., GAUROIS B., ECTORS F., TOUATI K.,and MASSIP A. Some factors affecting successfull vitrification of mouse blastocysts Theriogenology 1988,30,1177-1183 .

VANDERZWALMEN P., TOUATI K., ECTORS F.J., MASSIP A., BECKERS J.F.and ECTORS F. Vitrification of bovine blastocysts. Theriogenology 1989, 31, 270 .

TOUATI K., VANDERZWALMEN P., ECTORS F.J., BECKERS J.F. and ECTORS F. Low dose of FSH early in estrous cycle enhances superovulatory response in heifers. Theriogenology 1989, 31, 269.

MASSIP A., VANDERZWALMEN P., SCHEFFEN B. and ECTORS F. Some significant steps in the cryppreservation of mammalian embryos with a note on a vitrification procedure. Animal reproduction science 1989, 19, 117-129.

VANDERZWALMEN P., BERTIN G., SCHOYSMAN_DEBOECK A., GEERTS L., DEBAUCHE CH. and SCHOYSMAN R. Techniques de selection, amélioration, separation du sperme dans un programme de fecondation In Vitro Fertilità e sterilità , Atti del X corso di aggionamento 1989, 1, 89-120. Ed Cittadini, De cecco, Massi

VANDERZWALMEN P., BERTIN G., SCHOYSMAN_DEBOECK A., GEERTS L., DEBAUCHE CH. and SCHOYSMAN R. Techniques of sperm selection, improvement and separation in an in vitro fertilization program. Acta europea fertilitatis 1989, 20, 269-283.

SCHOYSMAN R., BERTIN G., VANDERZWALMEN P. and SEGAL L. Utilisation du sperme épididymaire dans un programme de FIV. Stérélité masculine et procréation médicalement assistées. Progrès en andrologie. Ed doin-paris 1989 117-148.

VANDERZWALMEN P., BERTIN G., DEBAUCHE CH., GEERTS L. and SCHOYSMAN R. Analyse morphologique des spermatozoïdes humain sélectionnés par centrifugation sur gradient de percoll ou par migration ascendante et application dans un programme de FIVET Bulletin de la sociéte d’andrologie de langue française. 1990, 4, 52-53.

VANDERZWALMEN P., BERTIN G., DEBAUCHE CH., GEERTS L. and SCHOYSMAN R. Spermatozoa morphology and IVF pregnancy rate: a comparison between Percoll gradient centrifugation and swim-up procedures. Hum. Reprod.1991, 6, 581-588.

VANDERZWALMEN P. BERTIN G. and SCHOYSMAN R Treatment of male infertility by partial zona dissection in an IVF program. Contracept. Fertil. Sex 1991 8, 324-326.

BARLOW P., VANDERZWALMEN P., SCHOYSMAN R. and LEROY F Improvement of fertilizing performance by normal and abnormal mouse semen after zona opening of mature oocytes. Molec. Reprod. Dev. 1991, 30, 385-389.

COGNAT B., GUERIN J.F;, MATHIEU C., SCHOYSMAN R , VANDERZWALMEN P. and BERTIN G. Prélèvement de spermatozoïdes épididymaires: Techniques et applications. Contracept. Fertil. Sex. 1991, 19, 763-769.

VANDERZWALMEN P, BARLOW P., NIJS M., BERTIN G., LEROY F. and SCHOYSMAN R. Usefulness of partial dissection of the zona pellucida in a human in-vitro fertilization programme. Hum. Reprod. 1992, 7, 537-544.

VANDERZWALMEN P, BERTIN G, GEERTS L, NIJS M, SEGAL L, SCHOYSMAN- DEBOECK, VAN ROOSENDAAL E and SCHOYSMAN E. Arguments in favour of the possible role of pentoxifylline in accelerating the capacitation process in human spermatozoa. Hum. Reprod 1992, supplement 1, 250.

SCHOYSMAN R, VANDERZWALMEN P. Towards the definition of the fertilizing spermatozoa. Acta Eur. Fert. 1992,23, 131-140.

BARLOW P., VANDERZWALMEN P., DELVIGNE A., VAN HOECK J., SCHOYSMAN R. and LEROY F. L’assistance à la fécondation de l’ovocyte humain par micromanipulation. Revue médicale de Bruxelles. 1992, 23-27.

VANDERZWALMEN P, BERTIN G, GEERTS L, NIJS M, SEGAL L, SCHOYSMAN- DEBOECK, VAN ROOSENDAAL E and SCHOYSMAN R. Semen defect:latest development and alternatives for handling male infertility by IVF. In: Andrology in the nineties. International symposium on male infertility and assisted reproduction, 1993, 60-68.

VANDERZWALMEN P, BERTIN G, NIJS M, SCHOYSMAN R Value of partial zona dissection for couples suffering from male subfertility. In: Proceedings European symposium on micromanipulation, 1993, 41-46.

VANDERZWALMEN P, BERTIN G, NIJS M, SCHOYSMAN R La micromanipulation appliquée aux gamètes et embryons humain dans le cadre d’un programme de fécondation in vitro. Référence en gynecologie obstétrique 1993,1, 347-359.

VANDERZWALMEN P, BERTIN G, NIJS M, SCHOYSMAN E. Cumulative effect of pentoxifylline and follicular fluid to enhance fertilisation after SUZI in case of extreme OAT. J. Ass. Reprod. Genet. 1993, 10, 118.

SCHOYSMAN R, VANDERZWALMEN P, NIJS P, SEGAL L, SEGAL-BERTIN G, GEERTS L, van ROOSENDAAL E, SCHOYSMAN-DEBOECK A. Pregnancy after fertilization with human testicular spermatozoa. Lancet, 1993, 342,1237.

NIJS M., GEERTS., VAN ROOSENDAAL E., BERTIN G., VANDERZWALMEN P and SCHOYSMAN R. Prevention of multiple pregnancies in an in vitro fertilization program. Fertil. Steril. 1993, 59, 6, 1245-1250.

NIJS M., VANDERZWALMEN P., BERTIN G., GEERTS., SEGAL L., VAN ROOSENDAAL E. and SCHOYSMAN R. A monozygotic twin pregnancy after application of zona softening on a frozen-thawed blastocyst: case report. Hum. Reprod. 1993, 8, 127-129.

SCHOYSMAN, SEGAL, VANDERZWALMEN, NIJS, BERTIN Assisted fertilizatio with epididymal spermatozoa. Acta Eur. Fert. 1993,24, 7-12.

NIJS M, BERTIN-SEGAL G, GEERTS L, SEGAL L, VANDERZWALMEN P, van ROOSENDAAL E, SCHOYSMAN R. Successful fertilization by testicular sperm in an IVF programme: case report. Hum. Reprod. 1993,8, supplement 1,11.

SCHOYSMAN R., VANDERZWALMEN P. , BERTIN G., NIJS M, SEGAL L. Oocyte fertilization by testicular spermatozoa in the human. Preliminary results. J;Ass. Reprod. Gen. 1993,10,103.

SCHOYSMAN R, SEGAL L, VANDERZWALMEN P, NIJS M, BERTIN G. Fertilisation ovocytaire par sperme épididymaire et testiculaire. Référence en gynécologie obstétrique. 1993,1,240-242.

SCHOYSMAN R, VANDERZWALMEN P, NIJS M, SEGAL-BERTIN G, van de CASSEYE M. Successful fertilization by testicular spermatozoa in an IVF programme Hum. Reprod. 1993,8,1339-1340.

VANDERZWALMEN P, LEJEUNE B, SEGAL L, VAN ROOSENDAAL E, SCHOYSMAN R. La micro-fécondation assistée de l’ovocyte humain. Médi-sphère gynécologie 1994,5, 14-18.

VANDERZWALMEN P, NIJS M, BERTIN G-SEGAL L, SEGAL L, SCHOYSMAN R (1994) Epididymal sperm aspiration for in vitro fertilisation. In: Microsurgery of male infertility. Ed: Fondazione per gli studi sulla riproduzione umana. (1994) 229-248.

VANDERZWALMEN P, RAICK D, MARECHAL D, NIJS M, SCHOYSMAN, BAECKELAND. Effect of pentoxifylline and follicular fluid on the acrosome reaction: an ultrastructural study. Hum. Reprod. 1994,9, supplement 4, 279.

VANDERZWALMEN P, BERTIN G, NIJS M, SCHOYSMAN E. Clinical results of intracytoplasmic sperm injection for severe male-factor infertility. Hum. Reprod. 1994, 9, supplement 4, 26.

SCHOYSMAN R, VANDERZWALMEN P, NIJS M, SEGAL-BERTIN G, van de CASSEYE M. Pregnancy obtained with human testicular spermatozoa in an in vitro fertilisation program. J. Androl. 15, 10-13,1994.

SCHOYSMAN R, LEJEUNE B, BERTIN G, NIJS M, VANDERZWALMEN P. Zwangerschap verwezenlijkt met testiculair sperma in een in vitro fertlizarie programma. Medi sfeer Gynaecologie 1994,36-38.

VANDERZWALMEN P, LEJEUNE B, BERTIN G, NIJS M, VAN DAMME B, SCHOYSMAN R. Fertilisation of an oocyte microinseminated with a spermatid in an in-vitro fertilisation programme. Hum. Reprod. 1995, 10, 502-503.

VANDERZWALMEN P, LEJEUNE B, NIJS M, SEGAL-BERTIN G, SCHOYSMAN R Fertilization ovocytaire par spermatide. Gyn. Obstet., 325,11,1995.

VANDERZWALMEN P, ZECH H, ZECH N, NIJS, BERTIN G, LEJEUNE B, SCHOYSMAN R Intracytoplsmic sperm injection with ejaculate, testicular and epididymal spermatozoa. Official journal of the russian association of Human Reproduction. 2, 52-60,1995.

VANDERZWALMEN P, LEJEUNE B, SEGAL L, VAN ROOSENDAAL E, SCHOYSMAN R. Fertilisation and pregnancy rates after intracytoplasmic sperm injection using ejaculated, epididymal and testicular spermatozoa. Eur. Board. Urol. (1995) 4, 170-175.

VANDERZWALMEN P, SEGAL L, BERTIN G, NIJS M, SCHOYSMAN E. Fertilisation after intracytoplasmic injection of a late stage spermatid. J. Ass. Reprod. Genet.,1995, 12, (supplement), 95.

VANDERZWALMEN P, BERTIN G, NIJS M, LEJEUNE B, SCHOYSMAN E. Is there a relation between the total number of motile spermatozoa in the ejaculate and the outcome of fertilizations and pregnancies after ICSI. J. Ass. Reprod. Genet.,1995, 12, (supplement), 187.

VANDERZWALMEN P, BERTIN G, NIJS M, LEJEUNE B, SCHOYSMAN E. Evolution of the epididimal (MESA) and testicular (TESA) sperm aspiration results using different micromanipulation procedures. J. Ass. Reprod. Genet.,1995, 12, (supplement), 187.

YEMINI M, VANDERZWALMEN P, MUKAIDA T, SCHOENGOLD S, BIRKENFELD A. Intracytoplasmic Sperm Injection, Fertilization and Embryo Transfer after Retrieval of Spermatozoa by Testicualr Biopsy from an Azoospermic Mals with Testicular Tubal Atrophy Fertil. Steril. 1995, 63, 1118-1120.

PRAPAS Y, PRAPAS N, HATZIPARASIDOU A, PRAPA S, NIJS M, VANDERZWALMEN P, VLASSIS G, JONES E. The echoguide embryo transfer maximizes the IVF results. Acta Europea fertilitatis (1995), 26, 113-115.

VANDERZWALMEN P, BERTIN G, NIJS M, LEJEUNE B, SCHOYSMAN R. Two essential steps for a successfull intracytoplsmic sperm injection: injection of immobilised spermatozoa after rupture of the oolema. Hum Reprod (1996),11, 540-547.

SCHOYSMAN R, LEJEUNE B, BERTIN G, NIJS M, VANDERZWALMEN P. New therapeutic possibilities handeling residual azoospermia. Acta Europaea Fertilitatis vol26, 4, 1996.

KAHRAMAN S, OZGUR S, ALATAS C, AKSAY C, BALABAN B, EVRENKAYA T, NUHOGLU A, NIJS M, VANDERZWALMEN P, SCHOYSMAN R High implantation and pregnancy rates with testicular sperm extraction and intracytoplamic sperm injection in obstructive and non-obstructive azoospermia Hum. Reprod. 1996 11, 673-676.

KAHRAMAN S, TASDEMIR M, TASDEMIR I, VICDON K, OZGUR S, POLA G, BIBEROGLOU K, VANDERZWALMEN P, NIJS M, SCHOYSMAN R Pregnancies achieved with testicular and ejaculated sperm in combination with intacytoplasmic sperm injection in males with totally immotile or initially immotile spermatozoa in the ejaculate. Hum. Reprod. 1996 11, 1343-1346.

KAHRAMAN S, OZGUR S, ALATAS C, AKSAY C, BALABAN B, EVRENKAYA T, NUHOGLU A, NIJS M, SCHOYSMAN R, VANDERZWALMEN P Fertility with testicular sperm extraction and ICSI in non obstructive azoospermic men Hum. Reprod. 1996, 11, 756-760.

ZECH H, VANDERZWALMEN P, BERTIN G, LEJEUNE B, NIJS M, RIEDLER I, STECHER A, VANDAMME B, SCHOYSMAN R Fertilisierungs-und schwangerschaftsrate nach ICSI mit ejakulierten, epididymalen und testikuleren spermatozoon J. Fertil. Reprod. 1996,3, 17-29.

SCHOYSMAN R, VANDERZWALMEN P, ZECH H, YEMINI M, BIRKENFIELD A, CHAN M, VANDAMME B, LEJEUNE B. Results of spermatid injection in human oocytes in relation to previous testis biopsy and oocytes treatment. American society for reproductive medicine (abstract) Boston, 1996, P358-S259.

ZECH H, VANDERZWALMEN P, NIJS M, LEJEUNE B, STECHER A, SCHOYSMAN R, Spermatozoon immobilization before ICSI: optimization of the procedure American society for reproductive medicine (abstract) Boston, 1996, P363-S261.

SCHOYSMAN R, LEJEUNE B, VAN ROOSENDAAL E, SEGAL L, VANDERZWALMEN P, NIJS M, VANDAMME B, BERTIN G Pregnancies obtained by microinsemination of round spermatids into oocytes in IVF-ICSI programs. Infertility World 1996, 7, 5.

NIJS M, VANDERZWALMEN P, VANDAMME B, SEGAL-BERTIN G, LEJEUNE B, SEGAL L, VAN ROOSENDAAL E, SCHOYSMAN R. Fertilizing ability of immotile spermatozoa after intracytoplasmic sperm injection. Hum. Reprod. 1996 11, 2180-2185.

VANDERZWALMEN P, ZECH H, BIRKENFELD A, YEMINI M, BERTIN G, LEJEUNE B, NIJS M, SEGAL L, STECHER A, VANDAMME B, VAN ROOSENDAAL E, SCHOYSMAN R. Intracytoplasmic injection of spermatids retrieved from testicular tissue: influence of testicular pathology, type of selected spermatids and oocyte activation. Hum. Reprod. 1997, 12, 6, 1203-1213.

VANDERZWALMEN P, DELVAL A, CHATZIPARASIDOU A, BERTIN G, ECTORS F, NIJS M, LEJEUNE B, PRAPAS N, PRAPAS Y, VAN DAMME B, SCHOYSMAN E. Pregnacies after vitrification of human day 5 embryos. Hum. Reprod. 1997 12, 98.

ECTORS F, VANDERZWALMEN P, VAN HOECK J, NIJS M, VERHAEGEN G, DELVIGNE A, SCHOYSMAN R, LEROY F. Relation of human follicular diameter with oocyte fertilisation and development after ICSI or IVF Hum. Reprod ,1997, 9, 2002-2005.

VANDERZWALMEN P, ET AL. (1998). The problems of spermatid microinjection in the human: the need for an accurate morphological approach and selective methods for viable and normal cells. Hum; Reprod. 13. 515-519.

VANDERZWALMEN P, ET AL. (1998). Vitrifikation von humanen embryonen in einem fortgeschrittenen entwicklungsstadium. J; fertilitat und reproduktion 2 20-26.

VANDERZWALMEN P. ET AL. (1999). Is there a future for spermatid injection? Hum. Reprod. 13. Supplement 4 71-84.

VANDERSCHUEREN D., SPIESSENS.C, KIEKENS C, VANDERZWALMEN P et al. Successful treatment of idiopathic anejaculation with electroejaculation after microsurgical vas aspiration. Hum. Reprod. 1998, 13, 370-371.

STECHER A, VANDERZWALMEN P. et al. Vitrification of the human embryo at an advanced stage of development. Official journal of the russian association of Human Reproduction. 4, 22-25 1998

ZECH H, VANDERZWALMEN P, PRAPAS Y, LEJEUNE B, DUBA E, SCHOYSMAN R Congenital malformations after intracytoplasmic injection of spermatids. Hum Reprod. 2000 Apr;15(4):969-71.

SCHOYSMAN R.R, ROOSENDAAL E, BOLLEN N., VANDERVORST M., VANDERZWALMEN P., STANDAERT V. BERTIN G, DEBAUCHE C, LEFERE CL. Modern sperm retrieval techniques and their usefulness in oocyte fertilization. BJU Int. 2001 Jul;88(2):141-6. Review.

NIJS M AND VANDERZWALMEN P. Role of testicular spermatozoa and spermatids. In infertility treatment. Eds Indira Hundaj. Oxford University Press. ( In press).

PRAPA Y, PRAPA N, HATZIPARASIDOU A, VANDERZWALMEN P, NIJS M, PRAPAS S, VLASSIS G. Ultrasound-guided embryo transfer maximizes the IVF results on day 3 and day 4 embryo transfer but has no impact on day 5. Hum Reprod. 2001 Sep;16(9):1904-8.

ZECH I, VANDERZWALMEN P, ZECH N Embryonalentwicklung nach ICSI bei “non male factor infertility” in relation zur injektionstechnik. 2002, J. fertilät Reprod. 4, 12, 13-18.

VANDERZWALMEN P, BERTIN-SEGAL G., DEBAUCHE CH., STANDAERT. V., VAN ROOSENDAAL E , VANDERVORST M., BOLLEN N.,., ZECH H., MUKAIDA T., TAKAHASHI K., SCHOYSMAN R. Births after vitrification at morula and blastocyst stages: effect of artificial reduction of the blastocoelic cavity before vitrification. Hum Reprod. 2002 Mar;17(3):744-51.

KAHRAMAN S, KUMTEPE Y, SERTYEL S, DONMEZ E, BENKHALIFA M, FINDIKLI N, VANDERZWALMEN P. Pronuclear morphology scoring and chromosomal status of embryos in severe male infertility. Hum Reprod. 2002 Dec;17(12):3, 193-200

ZECH N, VANDERZWALMEN P, STRECHER A AND ZECH N Vitrifikation von der eizelle bis zum blastozysten. 2002 J. fertilät Reprod. 12, 13-18.

VANDERZWALMEN P., BERTIN-SEGAL G., DEBAUCHE CH., STANDAERT. V., BOLLEN N., VANDERVORST M., VAN ROOSENDAAL E., SCHOYSMAN R. AND ZECH N. Vitrification of human blastocysts with the Hemi-Straw carrier: application of assisted hatching after thawing. Hum Reprod. 2003, Jul;18(7):1504-11.

VANDERZWALMEN P Application de la vitrification des blastocystes dans un programme d’AMP. Article publié pour les V èmes journées de la Société de Médecine de la Reproduction (SMR) Bordeaux 2003 (in press)

VANDERZWALMEN P , Delval A , ,Lejeune B , Puissant F Zech H . Vitrification: a promising method for the cryopreservation of human embryos. (Manuscript for the book on cryopreservation of gametes and embryos ESHRE 2004)

LIEBERMANN J, DIETL J, VANDERZWALMEN P, TUCKER M Recent developments in human oocyte, embryo and blastocyst vitrification: where are we now? Reprod Biomed Online. 2003 Dec;7(6):623-33.

SCHWARZLER P, ZECH H, AUER M, VANDERZWALMEN P, PFAU K, GOBEL G, ZECH N Pregnancy outcome after blastocyste transfer as compared to early cleavage stage embryo transfer. Hum Reprod. 2004 Sep;19(9):2097-102.

PRAPAS N , PRAPAS Y , PANAGIOTIDIS Y , PRAPA S, VANDERZWALMEN P , SCHOYSMAN R, MAKEDOS G GnRH agonist versus GnRH antagonist in oocyte donation cycles: a prospective randomized study. Hum Reprod. 2005 Jun;20(6):1516-20.

ZECH N, KOESTENBAUER S, VANDERZWALMEN P, SCHOONJANS L, DANLOY S, ZECH H, BLASCHITZ A, DOHR G . Paraffin-embedded manipulated blastocysts: a tool to demonstrate stem cell plasticity? Reprod Biomed Online. 2005 Mar;10(3):406-14.

KAHRAMAN S, SERTYEL S, FINDIKLI N, KUMTEPE Y, ONCU N, MELIL S, UNAL S, YELKE H, VANDERZWALMEN P. Effect of PGD on implantation and ongoing pregnancy rates in cases with predominantly macrocephalic spermatozoa. Reprod Biomed Online. 2004 Jul;9(1):79-85.

PRAPAS N , PRAPAS Y , PANAGIOTIDIS Y , PRAPA S, VANDERZWALMEN P , MAKEDOS G Cervical dilatation has a positive impact on the outcome of IVF in randomly assigned cases having two previous difficult embryo transfers. Hum Reprod. 2004 Aug;19(8):1791-5.

ZECH N, LEJEUNE B, ZECH H, VANDERZWALMEN P Vitrification of hatching and hatched human blastocysts: effect of an opening in the zona pellucida before vitrification Reprod Biomed Online. 2005 sept 11 (3):355 – 361.

KOESTENBAUER S , ZECH NH, JUCH H, VANDERZWALMEN P, SCHOONJANS L, DOHR G : Embryonic stem cells: similarities and differences between human and murine embryonic stem cells. Am J Reprod Immunol. 2006 Mar;55(3):169-80. Review.

VANDERZWALMEN P, ZECH N, GREINDL J, ECTORS F, LEJEUNE B Cryopreservation of human embryos by vitrification[ Gynecol Obstet Fertil. 2006 Sep;34(9):760-9

ZECH N, LEJEUNE B, PUISSANT F, VANDERZWALMEN S, ZECH H, VANDERZWALMEN P Prospective evaluation of the optimal time for selecting a single embryo for transfer: day 3 versus day 5. Fertil Steril. 2007 (88) 244-246

VATJA G; KUWAYAMA M; VANDERZWALMEN P Disadvantages and benefits of vitrification In “ Vitrification in human assisted reproduction: A user’s Manual and troubleshooting guide by MJ. Tucker and J Liebermann.

VANDERZWALMEN P; EBNER T; ZECH N One decade of experience with vitrification of human embryos in straws, hemi-straws, and high security vitrification straws In “ Vitrification in human assisted reproduction: A user’s Manual and troubleshooting guide by MJ. Tucker and J Liebermann.

VANDERZWALMEN P; ZECH H Selection of the best spermatozoa at high magnification: a way to improve the rate of development to the blastocyst stage before elective single embryo transfer (eSET) (in press)

MORTIMER S AND VANDERZWALMEN P Cryopreservation: to freeze or to vitrify? In: “Contemporary perspectives in assisted reproductive technology” eds GN Allahbadia and R Merchant. Elsevier, New Delhi, India (2006) 29, 265-282

S. KOESTENBAUER, P. VANDERZWALMEN, A. HAMMER, L. SCHOONJANS, S. DANLOY, H. ZECH, G. DOHR AND N.H. ZECH Apoptosis affects integration frequency: Adult stem cells injected in blastocysts show high caspase-3 activity • Cell Biol Int (2007) 31, 489 - 493

VANDERZWALMEN P, BACH M, NEYER T, STECHER A, ZINTZ M, ZECH N, Blastocyst formation after intracytoplasmic morphologically selected sperm injection (IMSI) according to the morphological integrity of human sperm nuclei RBMonline November 2008

UHER P, HÜTTELOVÁ R, KRÁLÍCKOVÁ M, NOVOTNÝ Z, ROKYTA Z, VANDERZWALMEN P, ZECH N. How can the haematopoietic stem cells from the umbilical cord blood be de-differentiated in vitro? Our first results using the co-cultivation systems. Ceska Gynekol. 2007 Aug;72(4):280- 3.

VANDERZWALMEN P, ECTORS F, GROBET L, PRAPAS Y; PANAGIOTIDIS Y, STECHER A, PUISSANT F, VANDERZWALMEN S, UHER P, Zech N. Aseptic vitrification of human blastocysts: development of an efficient exposure protocol to cryoprotectant solutions. (Part 1) Submitted to RBMonline

VANDERZWALMEN P, ECTORS F, GROBET L, PANAGIOTIDIS Y, PRAPAS Y; FRIAS P, STECHER A , LIEBERMANN J, VAN STEENBRUGGE A, VANDERZWALMEN S, ZECH N. Aseptic vitrification of human blastocysts: Application for blastocysts originating from infertile patients, egg donation and in vitro maturation (IVM). (Part 2) Submitted to RBMonline

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2. Abstract - Oral communications

MASSIP A, ECTORS F, DE COSTER R, D’IETEREN G, HANZEN C, VANDERZWALMEN P and DEFONSECA M. Deep freezing of cattle embryos in French straws. 9th Int.Congr. on Animal Reproduction and A.I. Madrid, 16-20 june 1980.

MASSIP A., MULNARD J. and VANDERZWALMEN P. In vitro cleavage of the cow egg from the 2-cell to the 16-cell stage.: a cinematographic analysis. International Congress, Annecy France 20-21-22 september 1982.

MASSIP A. and VANDERZWALMEN P. In vitro survival of mouse embryos frozen in glycerol or glycerol-sucrose. 8th Int.Meeting on problems of low T° biology. 1982 Leuven

MASSIP A and VANDERZWALMEN P. La vitrification, méthode d’avenir pour la cryoprotection des embryons. International Congress, Annecy June 1986

MASSIP A and VANDERZWALMEN P. Cryopreservation de l’embryon bovin: technique et résultats. 2° réunion de l’association européenne du transfert d’embryons Lyon, 5 septembre 1986.

PUISSANT F., MASSIP A., VANDERZWALMEN P. and LEROY F. Human pregnancy after replacement of embryos frozen at the compacted morula stage. Second meeting of the European Society of Human Reproduction and Embryology. 1986, pp 81.

MASSIP A and VANDERZWALMEN P Cryopreservation of half embryos by conventional and vitrification methods. Proceedings of Symposium: Application of egg and embryotechnologies to domestic animals. Copenhagen 1987.

BECKERS J.F., WOUTERS-BALLMAN P., VIVIERS-DONNAY I., DEMOUSTIER M, TOUATI K., VANDERZWALMEN P.,MASSIP A. and ECTORS F. Induction of superovulation in cattle. Réunion scientifique de l’association européenne de transfert embryonnaire. 1987

MASSIP A. and VANDERZWALMEN P Birth of twin calves after transfer of quarter embryos produced from morulae. Réunion scientifique de l’association européenne de transfert embryonnaire 1987

ECTORS F., VANDERZWALMEN P., MASSIP A., PSALTI Y., LOUMAYE E. and THOMAS K. Preliminary results in mouse oocytes vitrification. FRSM contact group on in vitro fertilization Belgian study group for fertility and sterility Bruxelles fevrier 1987

SCHOYSMAN R., SCHOYSMAN-DEBOECK A., VAN ROOSENDAEJ E., BERTIN G., VANDERZWALMEN P. and GRIJP L. Cryopreservation of sperm and its clinical application. International symposium on gametes physiology. November California 1988

ECTORS F.J., VANDERZWALMEN P., TOUATI K., DELVAL A., BECKERS J.F. and ECTORS F. In vitro maturation and fertilization of bovine oocytes. Joint meeting of the Belgian FRSM/FGWO contact group on IVF and the belgian study group for fertility and sterility. Bruxelles fevrier 1989

SCHOYSMAN R., VANDERZWALMEN P., BERTIN G. and GEERTS L. Results of a five year IVF program. XIII world congress on fertility and sterility. Marrakesh 1989

SCHOYSMAN R., BERTIN G., DEBAUCHE Ch., DEMOOR G., GEERTS L, GRIJP L., SCHOYSMAN_DEBOECK A., VAN BOSCH L., VANDERZWALMEN P. and VAN ROOSENDAAL E. Are fluctuation in results of an IVF program hapzard or explainable. XIII world congress on fertility and sterility Marrakesh 1989

BARLOW P., PUISSANT F., VANDERZWALMEN P., TRIGAUX Ph., VANDROMME J. and LEROY F. In vitro fertilization, development and implantation after visible light exposure of mature mouse oocytes. VII meeting contactgroup in vitro fertilization Fonds de la recherche medicale, groupe de contact fécondation in vitro. décembre 1990

NIJS M., VANDERZWALMEN P., BERTIN G., SEGAL L., VAN ROOSENDAAL E. and SCHOYSMAN R. Pregnancies obtained after zona softening of in vitro cultured or frozen-thawed human embryos. 8th international meeting of the european society of human reproduction and embryology. Den Haag, Nederland juillet 1992

BERTIN-SEGAL, GEERTS, NIJS, SCHOYSMAN-DEBOECK, SEGAL, VANDERZWALMEN, Van ROOSENDAAL, and SCHOYSMAN. L’énigme epididymaire: l’agénésie partielle. X congrès de la SALF, Nîmes 1992

VANDERZWALMEN P., BERTIN G., GEERTS L, NIJS M., SEGAL L., SCHOYSMAN R. Which alternative to choose in case of semen defect: SUZI and or PTF? Congrès de micromanipulation VUB Mars 1992. Poster selected for oral communication

NIJS M, GEERTS L, SEGAL-BERTING, SEGAL L, VANDERZWALMEN P, VAN ROOSENDAAL E, SCHOYSMAN R. Microsurgical aspiration of sperm from the vas deferens, the epididymis and the testis. Contactgroep IVF. Brussel, march1993

NIJS M, GEERTS L, SEGAL-BERTING, SEGAL L, VANDERZWALMEN P et al. Succesful fertilization by tezsticular sperm in an IVF program. ESHRE, Thessaloniki, 1994

VANDERZWALMEN P, BERTIN G, NIJS M, LEJEUNE B, SCHOYSMAN E. Two essential steps for a successfull ICSI: injection of immobilised spermatozoa after rupture of the oolema. Andrology in the nineties, 22, Genk october 1995.

VANDERZWALMEN P, BERTIN G, NIJS M, LEJEUNE B, SCHOYSMAN E Evolution of epididymal and testicular sperm extraction results using different micromanipulation procedures.Andrology in the nineties, 24, 1995.

VANDERZWALMEN P, BERTIN G, NIJS M, SCHOYSMAN E. Clinical results of intracytoplasmic sperm injection for severe male-factor infertility. Hum. Reprod. 1994, 9, supplement 4, 26.

NIJS M, LEJEUNE B, SEGAL-BERTIN G, SEGAL L, VANDERZWALMEN P, van ROOSENDAAL E, SCHOYSMAN R. Can pregnancy rates be maintained when two embryos are replaced in patients undergoing ICSI for severe OAT. Hum. Reprod. 1994, 9, supplement,4,40.

VANDERZWALMEN P, RAICK D, MARECHAL C, BERTIN G, NIJS M, SCHOYSMAN E, BAECKELAND Effect of pentoxifylline and follicular fluid on the acrosome reaction: an ultrastructural study Andrology in the nineties, 54, Genk october 1995.

VANDERZWALMEN P, SEGAL L, BERTIN G, NIJS M, SCHOYSMAN E. Fertilisation after intracytoplasmic injection of a late stage spermatid. J. Ass. Reprod. Genet.,1995, 12, (supplement), 95.

SEGAL L, VANDERZWALMEN P, BERTIN-SEGAL L,NIJS M, LEJEUNE B, SCHOYSMAN R. Progressieve verbetering van de resultaten door opvangen van epididymair en testiculair sperma in een IVF programma. Société royale Belge de gynécologie et d'obstétrique. Congrès national Bruxelles 1995

NIJS M, LEJEUNE B, SEGAL-BERTIN G, VANDAMME B, VANDERZWALMEN P, SCHOYSMAN B Fertilising ability of immotile sperm after ICSI Andrology in the nineties, 19, Genk 1995

NIJS M, SEGAL-BERTIN G, VANDERZWALMEN P, et al. Factors influencing the succes of ICSI. Panel on infertility, Ankara, 1995

KAHRAMAN S, OZGUR S, ALATAS C, TASDEMIR M, BALABAN B,BIBEROGLOU K, SCHOYSMAN R, VANDERZWALMEN P, NIJS M. Fertility with testicular sperm extraction and ICSI in azoospermic males. Andrology in the nineties, 50, Genk 1995

YEMINI M, VANDERZWALMEN P, MUKAIDA T, SCHOENGOLD S, BIRKENFELD A. Intracytoplasmic Sperm Injection, Fertilization and Embryo Transfer Following Retrieval of Spermatozoa by Testicualr Biopsy from Azoospermic Males with Testicular Tubal Atrophy . J. Ass. Reprod. Genet., 1995,12

ZECH H, VANDERZWALMEN P Fertilization Potential of Totally Immotile Ejaculated, Epididymal and Testicular Spermatozoa with and without Touching of the Tail J. Ass. Reprod. Genet., 1995,12

ZECH H, VANDERZWALMEN P Fertilization after ICSI with Severe OAT and Totally Immotile Spermatozoa J. Ass. Reprod. Genet., 1995,12

KAHRAMAN S, VANDERZWALMEN P, NIJS M, DELILBASI L, BALABAN B, BIBEROGLU K, Turkey’s First ICSI Applications and Results J. Ass. Reprod. Genet., 1995,12

NIJS M, VANDERZWALMEN P, VANDAMME B, SEGAL-BERTIN G, LEJEUNE B, SCHOYSMAN R. Fertilizing Ability of Immotile Sperm after Intracytoplasmic Sperm Injection J. Ass. Reprod. Genet., 1995,12

ZECH H, VANDERZWALMEN P Mikromanipulation an gameten. Journal fur fertilitat und reproduktion. 3, 58,1995

VANDERZWALMEN P, DELVAL A, CHATZIPARASIDOU A, BERTIN G, ECTORS F, NIJS M, LEJEUNE B, PRAPAS N, PRAPAS Y, VAN DAMME B, SCHOYSMAN E. Pregnancies after vitrification of human day 5 embryos. ESHRE Edinburgh 1997.

NIJS M, VANDERZWALMEN P, LEJEUNE B, SCHOYSMAN R Use of spermatids for ICSI Andrology in the nineties. third edition Norfolk 1997

NIJS M, SEGAL-BERTIN G, VANDAMME B, VANDERZWALMEN P, et al. In vitro culture of testicular spermatozoa: an improvement for the ICSI outcome. 10th World Congress on In Vitro Fertilization, Vancouver, 1997

PRAPAS Y, CHATZIPARADISOU A, VANDERZWALMEN P, NIJS M, PRAPAS N, DRAKOPOULOS A, LEJEUNE B, VLASSIS G, SCHOYSMAN R Retrieval of spermatozoa and spermatids from testicular biopsy in non obstructive azoospermic patients. ESHERE 1997 Edinburgh

ZECH H, STECHER A, ZECH N, VANDERZWALMEN P Effekt des transfers von metaphase II- Zystoplasma in germinal vesikel oozyten. Reproduktionsmedizin und endokrinologie Grazer congress 1998.

ZECH H, STECHER A, MURACH K, VANDERZWALMEN P Laser Assistiertes hatching- Ergebnisse einer prospektiv randomisierten studie. Reproduktionsmedizin und endokrinologie Grazer congress 1998.

STECHER A, ZECH H, ZECH I, VANDAMME B, VANDERZWALMEN P Embryonalentwicklung in definierten kulturmedien Reproduktionsmedizin und endokrinologie Grazer congress 1998.

NIJS M, VANDERZWALMEN P ICSI with immature sperm British Fertility Society Conference , Sheffield, 1998

VANDERZWALMEN P. ET AL. Pregnancy and implantation rates after transfers on day 4 and 5 of fresh and vitrified embryo. World congress of infertility society, Sydney 1999.

VANDERZWALMEN P. ET AL. (1999). Pregnancy and implantation rates after transfers of fresh and vitrified Morula and Blastocysts. First world conference of A PART Venice Italy 1999.

VANDERZWALMEN P., ZECH H., PRAPAS Y, PRAPAS N., NIJS M., VANDAMME B., SEGAL-BERTIN G., DEBAUCHE Ch., LEJEUNE B., VAN ROOSENDAAL E. SCHOYSMAN R; (1999) Pregnancy and implantation rates after transfers of fresh and vitrified embryos on day 4 or 5 J. Assist. Reprod. And Genetics 16, 147.

A.STECHER, H. ZECH, I. ZECH, M. NIJS, B.VANDAMME,VANDERZWALMEN P. (1999). Embryonalentwincklung in Sequentiellen Kulturmedien. J. Fertil., Repro. 1999.

H. ZECH, A. STECHER, N. ZECH, P. VANDERZWALMEN. (1999). Neue Techniken in der Reproduktionsmedizin: Effekt des Transfers von Methaphase II- Zytoplasma in Germinal-Vesikel-Oozyten. J. Fertil., Repro. 1999.

SANDER TH., ZECH N., STECHER A.,VANDERZWALMEN P , MURACH K., ZECH H. . Welche Volteile bringt eine Follikel-Stimulation tatsächlich? Salzburg Oktober 1999

ZECH N., VANDERZWALMEN P , STECHER A., MURACH K., ZECH H. Vorstellung neuer ideen zur Eizelrekrutierung. Salzburg Oktober 1999

STECHER A.,VANDERZWALMEN P , MURACH K., ZECH N., ZECH H. Schicksal von Germinal-Vesikel-Eizellen im stimulierten Zyklus. Salzburg Oktober 1999

ZECH H., VANDERZWALMEN P , MURACH K., STECHER A., ZECH N. Blastozysten-Kultur: State of the Art. Salzburg Oktober 1999

ZECH N., VANDERZWALMEN P , STECHER A., MURACH K., ZECH H. Vorstellung der “Schockwellentheorie” als möglicher schädigender Einfluss auf die Embryonen nach dem Kryokonservieren und Aftauen. Jahrestagung der Österreichischen Gesellschaft fûr Reproduktionsmedizin und Endokrinologie. Salzburg Oktober 1999.

P VANDERZWALMEN, ZECH H, T MUKAIDA, K TAKAHASHI , G BERTIN AND E VAN ROOSENDAAL , R. SCHOYSMAN Artificial reduction of the blastocoelic cavity increase the survival of blastocysts (BL.) after vitrification protocol. Fert. Steril. (suppl) 2000, 74 48.

VANDERZWALMEN P., BERTIN-SEGAL G, STANDAERT. V., ZECH I , ZECH H..SCHOYSMAN R. Outcome of human blastocysts after ultra-rapid vitrification in relation to the cleavage stage embryo quality. Proceeding of the Alpha congress New York , 2001.

ZECH H, VANDERZWALMEN P, ZECH N, PFAU K AND SCHWÄRZLER Blastozystenkultuur- praktisches vorgehen- untersuchungen zum „fetal outcome“. 2001, J. fertilät Reprod. 4, 39.

VANDERZWALMEN P, BERTIN-SEGAL G., DEBAUCHE CH., STANDAERT. V., BOLLEN N., VANDERVORST M., VAN ROOSENDAAL E., LEFERE C., STRECHER A., ZECH H.(2).SCHOYSMAN R.. Cryopreservation of oocytes and embryos: from conventional freezing procedure to vitrification.Assist. Reprod. And Genetics 2002 , 19 (suppl) 402.

VANDERZWALMEN P., BERTIN-SEGAL G., DEBAUCHE CH., STANDAERT. V., BOLLEN N., VANDERVORST M., VAN ROOSENDAAL E., LEFERE C. , STRECHER A., ZECH H. AND SCHOYSMAN R. Assisted hatching affects the pregnancy rate in vitrified blastocysts transfer cycles. Hum. Reprod. 2002 17, (suppl), 77. ESHRE Vienna

JEZIOROWSKI T, ZECH I, ZECH N AND VANDERZWALMEN P. Embryo development after ICSI and after standard IVF in sibling oocytes from non-male factor infertility patients- a comparison. Hum. Reprod. 2002 17, (suppl), 44. ESHRE Vienna

LEJEUNE B, SCHOYSMAN R, VANDERZWALMEN P Vitrification of blastocysts after artificial opening of the zona pellucida at the morulae stage. Belgian Study Group Of Reproductive Medicine. 2003.

STECHER A, VANDERZWALMEN P, ZECH N, ZECH H Culture of human embryos to day 5 in sequential medium or in Simplex Optimization culture media: a prospective analysis 2005 J.Reproduktionsmed. Endokrinol. 3, (in press)

VANDERZWALMEN P, GROBET L, ZECH N, ECTORS F.J Cryopreservation of murine embryos: in vitro comparison of slow freezing versus vitrification in FVB and C57Bl/6 (B6) strains 2005 J.Reproduktionsmed. Endokrinol. 3, 10-11

ZECH H, LEJEUNE B, PUISSANT F, ZECH N, VANDERZWALMEN S, VANDERZWALMEN P Single embryo transfer: selection on day 3 or day 5? A prospective observational study. 2005 J.Reproduktionsmed. Endokrinol. 3, (in press)

VANDERZWALMEN P, LEJEUNE B, STECHER A, ZECH N, DELVAL A, ZECH H Aspectical vitrification of day 3 and day 5 embryos in a hermetically closed container. 2005 J.Reproduktionsmed. Endokrinol. 3, 9-10

BACH M; NEYER T; STECHER A; VANDERZWALMEN P; ZINTZ M; ZECH H Morphological integrity of human sperm nuclei and blastocyst formation after intracytoplasmic morphologically selected sperm injection (IMSI) Hum. Reprod.. ESHRE lyon 2007

VANDERZWALMEN P Aseptic vitrification of mice and human blastocysts using Glycerol – PROH as cryoprotectant. Osterreichischen Gesellschaft fur Reproduktionsmedizin und Endokrinologie Insbruck september 2007

CASSUTO G, VANDERZWALMEN P How improve the rate of good quality blastocyst after ICSI regarding a new approach for classification of motile human spermatozoa. ASRM Washinghton 2007

3. Abstract - Poster

VANDERZWALMEN P., BERTIN G., DEBAUCHE Ch GEERTS L. and SCHOYSMAN R. Comparaison de 2 techniques de selection des spermatozoïdes et application dans un programme de FIV. VII congrès de la société d’andrologie de langue francaise. Bruxelles 1989.

VANDERZWALMEN P, LEJEUNE B, NIJS M, BERTIN G and SCHOYSMAN R. Facteurs influencant le taux de fécondation en micro-injection intra-cytoplasmique. Société d’Andrologie de langue Francaise: XI congrès Deauville 94.

VANDERZWALMEN P, ZECH H, STECHER B, LEJEUNE B, NIJS M, SCHOYSMAN R, BIRKENFELD A, YEMINI M Intracytoplasmic injection of spermatids retrieved from testicular tissue. XIVth Testis Workshop: Germ cell development, division, disruption and death. Baltimore, 1997.

BERTIN G., VANDERZWALMEN P., NIJS M., SEGAL L., VAN ROOSENDAAL E. and SCHOYSMAN R. Microsurgery of the infertile male and IVF: pregnancy achieved using sperm aspirated from the epididymis and vas deferens. The European society of human reproduction and embryology 8th international meeting Den Haag juillet 1992.

NIJS M, VANDERZWALMEN et al. Arguments in favour of the possible role of PTF in accelerating the capacitation processes in human spermatozoa. ESHRE Thessaloniki,1993.

LEJEUNE B, VANDERZWALMEN P ET AL. Reduced implantation rate after transfer of frozen-thawed embryos obtained by ICSI.

ESHRE, Edinburgh 1997.

LEJEUNE B, VANDERZWALMEN P ET AL. Implantation rate of embryos obtained using ejaculated, epididymal and testicular spermatozoa.

ESHRE, Edinburgh 1997.

PRAPAS Y, CHATZIPARASIDOU A, VANDERZWALMEN P.et al. Retrieval of spermatozoa and spermatids from testicular tissue in non-obstructive azoospermic patients. Andrology in the nineties, Genk, 1998.

VANDAMME B, NIJS M, VANDERZWALMEN P ET AL. (1998) Transport of oocytes prior to intracytoplasmic sperm injection: a comparative study. ESHRE, Goteborg,1998.

LEJEUNE B, VANDERZWALMEN P ET AL. (1998) Five year experience with ICSI using epididymal and testicular sperm cells. P 079

IFFS San Fransisco.

ZECH H., STECHER A. VANDERZWALMEN P ET AL. (1998) Investigation of the usefulness of Laser-assisted Hatching for women of ages above and before forty years. Poster P 969 IFFS San Fransisco.

NIJS M, BERTIN G, VANDERZWALMEN P et al. In vitro culture of testicular and epididymal spermatozoa: immotility is non-viability. Andrology in the nineties, Genk, 1998.

VANDERZWALMEN P, ZECH H, LEJEUNE B, NIJS M, VANDAMME B, PUISSANT F, SCHOYSMAN. Evaluation of developmental capacity of morula, early blastocysts, blastocysts and expanded blastocysts. Hum. Reprod. 1999 (14) 333. Tour 1999.

VANDERZWALMEN P, ZECH H, LEJEUNE B, NIJS M, VANDAMME B, PUISSANT F, SCHOYSMAN. Pregnancy and implantation rates after transfers on day 4 and 5 of fresh and vitrified embryos. Hum. Reprod. 1999 (14) 333. Tour 1999.

VANDERZWALMEN P, LEJEUNE B, ZECH H, NIJS M, VANDAMME B, PUISSANT F, SCHOYSMAN. Maternal age:a factor affecting huamn expanded blastocyste formation and implantation rate Hum. Reprod.1999 (14) 334. Tour 1999.

VANDERZWALMEN P., BERTIN G., DEBAUCHE V., STANDAERT V. and SCHOYSMAN R. In vitro” survival of metaphase II oocytes (MII) and blastocysts after vitrification in an Hemi-straw (HS) system. Fertil. Steril. (Suppl.) 2000 74, 215. American society for reproductive medicine San Diego.

VANDERZWALMEN P. Un nouveau concept pour la cryopreservation du blastocysts: la reduction de la cavité blastocèlique avant la vitrification. (2000) Journées de la FFER Reprod. Hum. Horm. XIII (supplément), 38.

VANDERVORST M., VANDERZWALMEN P., STANDAART V., DEBAUCHE C., BERTIN G., ZECH H., ZECH I., BOLLEN N., VAN ROOSENDAAL E. AND SCHOYSMAN R. Blastocyst transfer after vitrification in an Hemi-straw (HS) system. Hum. Reprod. 2001 153, (suppl), 44.

ZECH N, BERTIN G, DEBAUCHE CH, STANDAART V, VANDERZWALMEN P Pregnancies following transfers of hatched vitrified blastocysts. Hum. Reprod. 2003 18, (suppl), 419. Madrid .

VANDERZWALMEN P, LEJEUNE B, STECHER A, ZECH N, DELVAL A, ZECH H Aspectical vitrification of day 3 and day 5 embryos in a hermetically closed container. (2005) Accepted for American society for reproductive medicine meeting Montreal 2005.

ZECH H, LEJEUNE B, PUISSANT F, ZECH N, VANDERZWALMEN S, VANDERZWALMEN P Single embryo transfer: selection on day 3 or day 5? A prospective observational study. Accepted for American society for reproductive medicine meeting Montreal 2005.

VANSTEENBRUGGE A, VANDERZWALMEN P; VASTERSAEGHER C; PAUWELS P Does high concentration of cryoprotectant during vitrification of blastocysts present any risks? Hum. Reprod.. 22, i152, P387 ESHRE lyon 2007

ZECH N, BACH M, NEYER T, STECHER A, ZINTZ M, VANDERZWALMEN P Embryo development to day 5 and pregnancies after ICSI or intracytoplasmic morphologically selected sperm injection (IMSI) in patients with previous failure of implantation: a sibling study ASRM washington 2007

ZECH N, BACH M, NEYER T, STECHER A, ZINTZ M, VANDERZWALMEN P Blastocyst formation after intracytoplasmic morphologically selected sperm injection (IMSI) according to the morphological integrity of human sperm nuclei ASRM washington 2007